The aim of this article is to look throw the steps of historical development of nanoscience and, particularly, its implementation in medicine. Nanoscience refers to the study of particles which measure up to 100 nm. Prospective though it was, for a long time the nanoscience had only been empirical study and couldn’t find its practical use. Only in the end of 80’s — beginning of 90’s of ХХ century, due to numerous research dedicated to properties of nanoparticles, such branches of science as nanomedicine and nanopharmacology appeared and developed dramatically. Nowadays the nanoparticles are widely used in medicine: in diagnostics, regeneration medicine, for development of medicines with long-termed and selective action. Nowadays, countries all around the world, including Ukraine, invest large amounts of money in nanotechnology. Numerous scientific institutions in Ukraine are working on research aimed at the nanoparticles’ impact on the biological tissue under physiological and pathological conditions, developing of new and modifying of the existing medicines using nanotechnology.