In this paper, we present allele frequencies at 15 autosomal STR-loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, ТН01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, FGA), which form the multiplex system for PCR-amplification “AmpFiSTR®Identifiler” (“Applied Biosystems”, USA), in population (n=241) of Odesa and Odesa region. The combined power of discrimination (PD) for the 15 tested STR-loci in investigated population was 0,999999999999999988. The data obtained for allele and genotype frequencies in this population conformed to Hardy — Weinberg expectations. According to the presented data loci D21S11, D2S1338, D18S51, FGA are the most informative markers for investigated population. The obtained data may be used as reference database for forensic-medicine laboratories of Ukraine.