It was studied the efficiency of experimental bacterial keratitis therapy by silver nanoparticles or decamethoxin using in rabbits. The effectiveness of experimental therapy increased in order: films with decamethoxin ® films with nanoparticles of silver 30 nm, obtained by electrical-beam evaporation ® instillation of silver 30 nm nanoparticles colloidal solution ® films with silver 30 nm nanoparticles, obtained by citrate method. The using of films with silver nanoparticles obtained by citrate method led to a shortening of cornea healing periods, reduction of severity and duration of inflammatory reaction, acceleration of conjunctival cavity sanitation, compared with other experimental groups.