Effect of antidisbiotic drugs on the liver condition in rats after anti- helicobacter therapy

Abstract

The purpose: in experiment to determine the hepatoprotective effects of phytogels "Kvertulin" and “Kvertulion” during anti-Helicobacter pylori therapy by defining the liver condition by hepatic enzymes indexes and the degree of dysbiosis in rats. Materials and methods: Two series of experiments were carried out. I series – 30 white rats of the Wistar line (females, 10 months, average weight 300 g). The animals were divided into 3 groups: 1 - control (norm), got a complete bio-feed, animals of the 2nd and 3rd got additionally per os mixture of antihelicobacter therapy drugs for 8 days. The 3rd group animals received applications of phytogel “Kvertulin” on the oral mucosa from the first day of the experiment during 11 days. In the II series of experiments, 30 rats with similar characteristics were also used. The animals were divided into 3 equal groups: 1- control (norm), animals of the 2nd and 3rd got additionally per os mixture of antihelicobacter therapy drugs for 8 days (omeprasol -1.3 mg/kg; augmentin – 50 mg/kg; clarithromycin -7.5 mg/kg). The 3 rd group animals received applications of phytogel “Kvertulidon” on the oral mucosa from the first day of the experiment during 11 days. Results: The number of leukocyte cells (percentage of neutrophils and lymphocytes) was determined in the blood of rats and the immunodeficiency index (lymphocyte / neutrophil ratio) was calculated. The level of hepatic markers was also determined: the activity of alanine aminotransferase and alkaline phosphatase enzymes, the level of inflammatory markers (malonic dialdehyde content and elastase activity), the activity of protective enzymes (lysozyme and catalase). In the liver homogenate (50 mg / ml 0.05 M Tris-HCl buffer pH 7.5) the content of malonic dialdehyde, the activity of elastase, alkaline phosphatase, catalase, lysozyme and urease (indicator of microbial contamination) was determined. The antioxidant-prooxidant index was calculated from the ratio of catalase activity and malonic dialdehyde content, and from the ratio of relative urease and lysozyme activities the degree of dysbiosis by Levitsky was calculated. Conclusions: The signs of primary and secondary liver damage due to intestinal dysbiosis were detected. The positive effect of phytogel “Kvertulidon” applications on the oral mucosa at the background of antihelicobacter therapy due to its antioxidant, immunostimulatory and prebiotic properties was determined. The data obtained provide a basis for clinical studies of the therapeutic and prophylactic effects of oral applications of phytogel "Kvertulidon" in patients who got antihelicobacter therapy in order to prevent or reduce the development of its adverse effects..