Abstract:
Non-alcoholic steatohepatitis (NASH) is a consequence of the progression of nonalcoholic fatty liver disease (NAFLD), which can lead to cirrhosis or hepatocellular
insufficiency. NAFLD is a predictor of cardiovascular diseases (CVD) development. The
important factor of the latter development is atherogenic dyslipidemia, and this is a type of
20-80 % NASH patients. The objective: to study the effectiveness of platelet-enriched
plasma (PRP) influence on the morpho-functional state of liver in rats with non-alcoholic
steatohepatitis. Materials and methods. The study was carried out on 80 adult male Wistar
rats. Animals were divided into three groups: I group - animals that received an atherogenic
diet for 90 days (n=10); II group - animals with simulated NASH and DL which received a
normal diet for 30 days (n=30); III group - animals with simulated NASH and DL and PRP correction (n=30); group of intact animals (n=10). NASH was simulated by introducing an
atherogenic diet, which consisted of lard and 50 g / kg of butter for 90 days. PRP was
punctured in the tissue of liver, twice at 0.05 ml with 7 days interval. The animals were taken
out of the experiment after 90 days of the atherogenic diet and on the 30th day after its
cessation or PRP injection. The indicators of body mass, liver mass index (LMI), lipogram,
hepatic transaminases were determined, and the further pathological examination of the liver
tissue was done. Results and discussion. On the 90th day of the atherogenic diet, the GH
level was 3.19 ± 0.56 mmol/l, ALT activity was 118 ± 6.12 U/l, AST 86 ± 4.52 U/l,
morphological signs of NASH were detected. On the 30th day, the lipid profile of group II
rats did not have statistical differences from group I, in rats after correction of NASH with the
use of PRP the level of LLD decreased by 51%, ALT activity - by 54%, AST - by 51%
compared to control group (p <0.05), morphologically revealed I - II degree steatosis, focal
protein dystrophy. Conclusions. On the 90th day of an atherogenic diet NASH was formed in
experimental animals. They had dyslipidemia, which progressed for at least 30 days while
maintaining a usual diet. After correction with PRP the level of atherogenic lipoproteins was
significantly decreased in rats with NASH and DL; the activity of liver enzymes was lower
compared to the group with simulated NASH and DL and the group with progression of
NASH and DL for 30 days, morphologically the decrease in liver steatosis and severity of
protein dystrophy comparing with a group with simulated NASH and DL and a group with
progressive NASH and DL was observed for 30 days.
