Abstract:
Difficulties of prevention and treatment of diseases of periodontal tissues, oral mucosa with metabolic syndrome make it an important problem of dentistry. The development of the new oral hygienic drug for improving the treatment and prevention of periodontal tissue diseases, oral mucosa is considered well timed. Purpose of research: To investigate safety of the new product for oral cavity and determine the effect on regenerative processes under conditions of the experimental trauma. Materials and methods: The study of acute toxicity of the new hygienic preparation in the form of elixir was performed.
The elixir was administered intragastrally to white nonlinear mice. Timeframes of intoxication development and death of animals registration was obligatory. Acute toxicity was investigated by a single application on the skin mice. Subacute (chronic) toxicity was studied when elixir was applied to the skin of mice for one month. The study of subacute toxicity was performed on white rats of Wistar line with intragastric administration for 60 days. The condition of the animals was evaluated by their appearance and behavior, as well as by a number of objective indicators (live weight, analysis of cellular blood composition, hemoglobin level, blood serum biochemical parameters). The study of reparative activity of the new elixir was carried out on an experimental model of the wound process–a round aseptic plane wound in rats. Results: Oral administration of the new product in different concentrations to mice didn’t result in death of animals in any case. According to obtained results the investigated agent isn’t toxic. All the tested biochemical parameters of blood serum were not significantly different from the control, indicating that there were no metabolic disorders. Study of subacute toxicity did not reveal any abnormalities as compared with the control animals. Positive dynamics of wound healing process was revealed visually in animals that received topical application of hygienic products based on propolis, which was confirmed by a quantitative calculation by the planimetry method. The difference at the area of wound defect of the animals, which were made application with the new product, and in rats of the control and comparative groups was determined on the 3rd, 7th, 14th days of the experiment. The difference in changes at the wound area in rats of the main and comparative group was on the 3rd day–25.7%, on the 7th day–37.5% and on the 14th day – 45.2%. Conclusions: a complete safety of the new hygienic product for the oral cavity–a tooth elixir on the basis of biologically active substances of bee products and compounds of plant origin–during studies of acute and subacute toxicity was determined. Reparative activity of the new product for oral cavity was determined on the model of wound process, which is better in drug of comparison “Phytopropolis”.
