Abstract:
Nowadays the development of novel biomaterials which would promote tissue lesions
regeneration is one of the crucial tasks of regenerative medicine. In the current research we
developed a biological active membrane composed of decellularized, lyophilized human
amniotic membrane in combination with growth factors of platelet-rich plasma (PRP) and
hyaluronic acid. On the basis of histological examination, scanning electron microscopic
examination and DNA content analyses it was drawn a conclusion that Sodium Dodecyl
Sulfate (SDS) (Sigma) solution and 1% Triton X-100 solution completely remove all existing
cells of placenta and chorionic-amniotic membrane without impairment of their structure.
After the processes of decellularization and lyophilization the DNA content was less than 2%. The PRP growth factors as the components biological matrix provide faster regeneration. The
latter are fixed on the surface of cellular structure of decellularized, lyophilized human
amniotic membrane by the reservoir of gelatinous hyaluronic acid.
